Gongjindan enhances hippocampal memory in a mouse model of scopolamineinduced amnesia. Loganin improves learning and memory impairments induced. Thirtysix male balbc mice weighing around 2535 g were employed in the present investigation. Electroencephalographic characterization of scopolamine induced convulsions in fasted mice after food intake asiye nurtena, ilknur ozena, sacit karamursela,b, ihsan karaa aistanbul university, institute for experimental medical research, department of neuroscience. To investigate the potential role of ramipril and losartan in preventing scopolamineinduced memory impairment in rats. The objective of the present study was to evaluate the nootropic, neuroprotective and neurotrophic roles of phl in the subacute scopolamine induced amnesia in mice. Scopolamine induced amnesia has been proposed as a model for dementia where reduced cholinergic function is the suspected cause. Neuroprotective effects of meloxicam and selegiline in.
Passive avoidance paradigm was used to assess learning and. Two time windows of anisomycininduced amnesia for inhibitory. Antiamnesic effects of a hydroethanolic extract of crinum. In the present study we investigated the antiamnesic activity of vitex negundo in scopolamine induced amnesia in rats. Electroencephalographic characterization of scopolamine. Antiamnesic effect of aqueous extract of crataeva nurvala. Peripheral electrical stimulationinduced suppression of. However, according to current clinical dosage and treatment period, its direct effect on memory is unclear. Synergistic effects of galantamine and memantine in attenuating scopolamineinduced amnesia in mice. A rtpcr analysis showing bdnf 692 bp, gfap mrna 641 bp and internal control gapdh 108 bp.
To determine whether sulforaphane can preserve cognitive function, we examined its effects on scopolamine induced memory impairment in mice using the morris water maze test. Pdf synergistic effects of galantamine and memantine in. Shortterm memory deteriorated in the mice with scopolamineinduced amnesia, concomitant with enhanced ache expression and. After 7 days of acclimatization period, animals were trained in morris water maze test.
The mice had access to water and food pellets cargill agri furina, gyeonggido, korea ad libitum, and were housed in a room maintained at 23 2c with a 12h. The effect of the water extract of anoectochilus formosanus afw on scopinduced amnesia was investigated in a stepthrough passive avoidance task in rats. Pass assisted prediction and pharmacological evaluation of. Therefore, in the present studies, we used this animal model of ad, in which we provoked scopolamine induced memory deficits, to investigate the procognitive. Deb, debasree and nayak, veena and bairy, kl and rao, mohandas kg and shetty, jeevan and hegde, mangala v and koshy, salini s 20 antiamnesic and neuroprotective effects of low dose of ramipril and losartan in scopolamine induced amnesia model of dementia. Protective effects of ralipoic acid xu cui,1 pingping zuo,2 qing zhang,2 xuekun li,2 yazhuo hu,1 jiangang long,3 lester packer,4 and jiankang liu3,5,6 1institute of gerontology and geriatrics, chinese pla general hospital, beijing, peoples republic of china. Effect of aecn on scopolamineinduced retrograde amnesia to study the effect of the drug extract on retrieval or retention, the animals received the vehicle during the acquisition trial days 14 and were subjected to four consecutive trials per day for four consecutive days to locate the platform in the centre of the water tank. Purpose we have observed that the commonly used ketaminexylazine anesthesia mix can induce a focally severe and permanent corneal opacity. The band intensity was quantitated densitometrically represented as. Antiamnesic and neuroprotective effects of low dose of.
Amnesia was induced with scopolamine 1 mgkg, ip in the mice and behavioral tests were started 30 min after the injection. Terminalia chebula extract prevents scopolamineinduced amnesia via. Sulforaphane, an organosulfur compound present in cruciferous vegetables, has been shown to exert neuroprotective effects in experimental in vitro and in vivo models of neurodegeneration. Electroencephalographic characterization of scopolamineinduced convulsions in fasted mice after food intake asiye nurtena, ilknur ozena, sacit karamursela,b, ihsan karaa aistanbul university, institute for experimental medical research, department of neuroscience, 34280, istanbul, turkey. In this study, we adopted a mouse model of scopolamineinduced.
Effect of aecn on scopolamine induced retrograde amnesia to study the effect of the drug extract on retrieval or retention, the animals received the vehicle during the acquisition trial days 14 and were subjected to four consecutive trials per day for four consecutive days to locate the platform in the centre of the water tank. The present study was undertaken to investigate the in vivo ability of hydroethanolic crude extract of crinum macowanii to ameliorate memory impairment induced by scopolamine. Terminalia chebula extract prevents scopolamineinduced amnesia. Protection from amnesia by pretraining but not preexposure to the task apparatus joao quevedo 1, 3, 4. Thus, scopolamine induced amnesia is a very well established animal model of memory dysfunction, widely used to test potential drugs of antialzheimers properties kanwal et al. John college of pharmacy, yellapur, hasanparthy, warangal506371. The first experiment was designed to evaluate the effects of pretraining. Neuroprotective effects of soy isoflavone s on scopolamineinduced am nesia in mice article pdf available in nutrients 107. Nootropic, neuroprotective and neurotrophic effects of phloretin in scopolamine induced amnesia in mice. On scopolamine induced cognitive dysfunction in mice krishna mohan chinnala, swetha pinninti, madhan mohan elsani department of pharmacology, st. Peripheral electrical stimulationinduced suppression of morphineinduced ccp in rats. Scopolamine 3 mgkg ip given before an acquisition trial, reduced the retention of a onetrial passive avoidance step through response in mice. Genistein ameliorates scopolamineinduced amnesia in mice. Evaluation of neuroprotective effect of quercetin with donepezil in.
Effects of imperatorin on scopolamineinduced cognitive. After prediction of aforementioned activities, the study was carried out in vivo using scopolamine induced amnesia model in mice. The antiamnesic effect of afw on the scop induced amnesia was significantly reversed by a peripheral. Vinpocetine is an inhibitor of phosphodiesterase type 1 pde1, which has been used for treating stroke for over 40 years. May 14, 2015 we evaluated the neuropharmacological effects of 30% ethanolic pine needle extract pne on memory impairment caused by scopolamine injection in mice hippocampus. Memory impairment was induced in male wistar rats by administration of scopolamine 1 mgkg intraperitoneally. Reversal effect of amphetamine on cognitive deficit induced by scopolamine in the. Effect of resveratrol on scopolamineinduced cognitive. Effect of scopolamine on bdnf and gfap expression in mi. Terminalia chebula extract prevents scopolamine induced amnesia via. Scopolamine administration may be considered as a psychopharmacological model of alzheimers disease ad. The animal model of scopolamine scopinduced amnesia is widely used to. Alzheimers disease ad is known as the leading cause of dementia amongst people aged 65. Memory impairment was induced by scopolamine 3 mgkg, i.
They wont be returned if another induced amnesia is put into a graveyard, even if that induced amnesia is represented by the same card. Elevated plus maze the elevated plus maze consisted of two open arms. Suspecting that this might have something to do with childhood amnesia, the researchers designed some experiments in mice and other animals which. The mice were orally pretreated with ge 10, 20 and 40 mgkg and donepezil 1. Antiamnesic activity of vitex negundo in scopolamine induced. Antidepressant effects of ketamine on depressionlike. Learning and memory were evaluated using elevated plus maze, y maze and morris water maze after the oral administration of two safe doses 200 mg\kg and 400 mg\kg of ethanolic extract of vigna mungo linn.
Effects of nootropic drugs in a scopolamineinduced. Amnesia induced by scopolamine has been widely used to understand the biochemical and behavioral changes in rodents. Rectangular maze test, morris water maze test, locomotor activity, and pole climbing test were conducted to evaluate the learning and memory parameters. Treadmill exercise ameliorates shortterm memory disturbance in. We evaluated the neuropharmacological effects of 30% ethanolic pine needle extract pne on memory impairment caused by scopolamine injection in mice hippocampus. Enduring amnesia induced by icv scopolamine is reversed by. Effect of scopolamine on bdnf and gfap expression in mice brain. Here, we examine whether norepinephrine ne, one of the most potent stress related hormones, leads to the difference in the efficacy of. We have investigated the neuroprotective and memory enhancing effect of 6gingerol gin, a pungent ingredient of ginger, using an animal model of amnesia. A significant antiamnesic effect was also found with nootropic drugs such as piracetam and ucb l059. Tce 100 or 200 mgkg was orally administered to mice for fourteen days days 114, and scopolamine. Pittel, who was a nationally known forensic psychologist and pioneer of research on the drug culture of san francisco, wrote that reports of date. Role of chiral chromatography in therapeutic drug monitoring and in clinical and forensic toxicology. May 09, 2014 suspecting that this might have something to do with childhood amnesia, the researchers designed some experiments in mice and other animals which have similar types of neurogenesis to learn more.
Prediction of probable antidementia and free radical scavenging activities pa of compounds and their inactivity for the mentioned effects pi using pass software. Extract of crinum macowanii on scopolamineinduced memory impairment in mice. To study acquision, consolidation and recall stages of memory, aterials and methods. Vinpocetine improves scopolamine induced learning and. The animals were then trained in the spatial learning task for 6 trials per day for 5 consecutive days. Sunitinib alone exhibits satisfactory efficacy in several mouse homografts and xenografts but unsatisfactory efficacy in many kinds of solid tumors in clinic. Exogenous norepinephrine attenuates the efficacy of. Behavioral experiments, including open field, ymaze, and fear conditioning tests were used to determine the possible role of vinpocetine on scopolamineinduced memory dysfunction. Chronic systemic dgalactose exposure induces memory. Jci longterm potentiation decay and memory loss are.
Study of neurogenesis in mice may have solved mystery of. Dose and time dependent effects of scopolamine sc 1, 3, 6, 10 mgkg bw on the level of expression of bdnf and gfap mrna in mouse cerebrum. Two time windows of anisomycininduced amnesia for inhibitory avoidance training in rats. Taken together, this study strongly showed that gjd exerted antiamnesic actions in a mice model of scopolamineinduced amnesia. Thirtysix male balbc mice weighing around 2535 g were employed in the. Scopolamine administration modulates muscarinic, nicotinic and nmda receptor systems soheil keihan falsafi1. Vehicletreated mice showed shorter pathlength as scopolaminetreated mice figure 2b. Nootropic, neuroprotective and neurotrophic effects of. Each animal received session of 15 trials with inter trial duration of 15 s for 5 days.
Doing a bit of googling i found this article on drug induced retrograde amnesia. Crinum macowanii has been found to contain alkaloids that have activity against acetylcholinesterase enzyme in vitro. If induced amnesia leaves the battlefield but isnt put into a graveyard, the exiled cards are lost forever. We report seven experiments in young adult mice which examine scopolamines effects on memory retention and whether its amnestic effects are specifically blocked by cholinergic agonists or cholinomimetics. International journal of pharmtech research coden usa. In this study, we investigated whether vinpocetine could reverse the scopolamine scoinduced cognitive deficits in animals. Moreover, nmdainduced modulation of morphine analgesia is influenced by sex, site of analgesia, morphine dose and time after injection 28. Amelioration of cognitive deficit by embelin in a scopolamine. Effects of erythropoietin on memory deficits and brain.
According to the wiki, retrograde amnesia is usually caused by trauma to the head. Dear peter, hypnosis is certainly the most familiar way of inducing amnesia, but not the only way. However, there is no report about the effects of ge on scopolamine scop induced amnesia in mice. Learning and memory were assessed by three separate tests. Gongjindan enhances hippocampal memory in a mouse model of. After 1 h of incubation in 5% fatfree dry milk, the membranes were. Chronic systemic dgalactose exposure induces memory loss. In this study, we used immunohistochemistry and western blot methods to weekly detect the biological behaviors of nerve cells in the hippocampal dentate gyrus of adult mice that received intraperitoneal administration of scopolamine for 4 weeks. Longterm administration of scopolamine interferes with. Antiamnesic effect of embelin in rats with scopolamineinduced amnesia.
Effects of nootropic drugs in a scopolamineinduced amnesia. Deficits induced by scopolamine in 3 mouse strains as assayed in the tmaze. It is seen also with slow acting parenteral general anaesthetics. Here, we studied a group of healthy elderly under scopolamine to test whether it. The purpose of this study was to establish the clinical and histological features of this deleterious side effect, its sensitivity with respect to age and anesthesia protocol, and approaches for avoiding it.
Plant extracts active properties effective on scopolamineinduced. Interaction of memantine and ketamine in morphine and. Enduring amnesia induced y icv scopolamine is reversed y sesame oil in male rats acta cirurgica brasileira vol. We investigated the effect of loganin on learning and memory impairments induced by. Antiamnesic effects of gjd in the radial arm maze task. A role for dopamine in the nucleus accumbens yaoying maa,b,c,d,e, xiangdang shia,b,c,d, jisheng hana,b,c,d, cailian cuia,b,c,d. Neuroprotective effect and molecular mechanism of 6. Kshirsagar1 1sudhakarrao naik institute of pharmacy, pusad,m. Male and female icr mice 9 weeks old were purchased from slc japan hamamatsu, shizuoka, japan. Effect of seabuckthorn extract on scopolamine induced. Gongjindan enhances hippocampal memory in a mouse model. Research journal of pharmaceutical, biological and chemical sciences, 4 1. The brains of a subset of animals from each group was.
The underlying mechanisms involve the regulation of the hippocampal cholinergic system and neurogenesis via activation of bdnf. Untrained mice did not learn the task but were spending the same time in the maze without any reward. It may be concluded that erythropoietin exerts a beneficial effect in memory deficits of mice possibly through. All the treatment groups are compared with scopolamineinduced amnesia, normal control group table 1. The present study deals with the evaluation of potential effects of eclipta alba ea in memory impairment of mice. One day before the test, each rat was placed in the pool for 60 s.
Loganin improves learning and memory impairments induced by. The effect of the water extract of anoectochilus formosanus afw on scop induced amnesia was investigated in a stepthrough passive avoidance task in rats. Scopolamineinduced amnesia has been proposed as a model for dementia where reduced cholinergic function is the suspected cause. Wistar rats 180200 g were trained on active avoidance task. Different from animals, receiving a diagnosis of cancer impacts chronic stress on patients. The antiamnesic effect of afw on the scopinduced amnesia was significantly reversed by a peripheral. Sustained inhibition of brotizolam induced anterograde amnesia by norharmane and retrograde amnesia by lglutamic acid in mice. Amnesia may be therapeutic for medical treatment or for medical procedures, or it may be a sideeffect of a drug, such as alcohol, or certain medications for psychiatric disorders, such as benzodiazepines.
I dont know much about neuroscience so i was wondering if anyone could make out anything from that excerpt. Therefore, the present study was carried out to investigate the beneficial effects and potential mechanism of ge against scop induced deficits in mice. The mice were housed in groups of three or five per cage under a controlled 1212hour lightdark cycle lights on from 7. However, there is no report about the effects of ge on scopinduced amnesia in mice. Animal model of alzheimers disease scopolamine induced. Bacopa monniera exerts antiamnesic effect on diazepaminduced anterograde amnesia in mice. All animals have free access to regular chow and water and were kept. Comparative effect of felbamate and other compounds on nmethyldaspartic acid induced convulsions and lethality in mice. One thing to be noted about this phenomenon is that amnesia is more successfully induced the more suggestible the subject is thus, false memories can be induced under the same process.
A single administration of cholinergic agonists such as oxotremorine, bm5, or arecoline, antagonized this amnesic effect of scopolamine. In the present study, the effects of meloxicam, selegiline, and coadministration of these drugs on scopolamineinduced learning and memory impairments in mice were investigated. In the mtm vehicletreated mice learned the task expressed as latencies in seconds, in contrast to scopolamine treated animals figure 2a. Memory impairment was induced by intraperitoneal administration of scp 1. Selective acheis, free of doselimiting side effects, are until now not available. Effect of scopolamine on bdnf and gfap expression in mi openi. Chronic systemic dgalactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice. Effect of seabuckthorn extract on scopolamine induced cognitive impairment dharam paul attrey1.
Twentyfour hours before spatial training, the animals were allowed to adapt to the maze for a 120second free swim. Therefore, the present study was designed to evaluate whether pre ecs administration of ccbs like verapamil, diltiazem, nifedipine, nimodipine, flunarizine and cinnarizine, can reduce retrograde amnesia induced by ecs in rats. Loganin is an iridoid glycoside found in the flos lonicerae, fruit cornus, and strychonos nux vomica. Antiamnesic activity of vitex negundo in scopolamine. Attenuation of scopolamine induced amnesia in mice. Aug 02, 2016 onehundred twenty specific pathogen free balbc male mice 10 weeks old. Onehundred twenty specific pathogenfree balbc male mice 10. The main animal model used in cholinergic theory research is the scopolamineinduced memory loss.